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They are best known for the role they played during the line inspection, in which they employed unusual techniques such as the use of the buttonhook to examine aliens for signs of eye diseases (particularly, trachoma) and the use of a chalk mark code. Symbols were chalked on the clothing of potentially sick immigrants following the six-second medical examination. The doctors would look at the immigrants as they climbed the stairs from the baggage area to the Great Hall. Immigrants' behavior would be studied for difficulties in getting up the staircase. Some immigrants entered the country only by surreptitiously wiping the chalk marks off, or by turning their clothes inside out.^[29]

The symbols used were:

• B – Back
• C - Conjunctivitis
• CT – Trachoma
• E – Eyes
• F – Face
• FT – Feet
• G – Goiter
• H – Heart
• K – Hernia
• L – Lameness
• N – Neck
• P – Physical and Lungs
• PG – Pregnancy
• S – Senility
• SC – Scalp (Favus)
• SI – Special Inquiry
• X – Suspected Mental defect
• X (circled) – Definite signs of Mental defect